David Florian (aka Dr. D-Flo) is an engineer with a passion for teaching and the open-source community. He created the Dr. D-Flo YouTube channel to share all of his projects and hopefully, inspire the next generation of DIYers. You can click through the tabs below to learn about David’s unique skillset and connections.
Modeling Diseased Bone Marrow
David is a Cancer Researcher at Vanderbilt University. His work focuses on using additive manufacturing to generate models that recapitulate hematological and solid cancer metastases in the bone marrow. By seeding the appropriate cell populations on 3D printed bone scaffolds, healthy and pathological marrow tissue can be generated outside of the body for screening drugs.
As a side project, David has developed low-cost CNC liquid handlers to automate and expedite his research. The designs and software for this affordable instrument are published by Scientific Reports and available online at openliquidhandler.com.
Biodegradable Metallic Implants
As an undergraduate researcher, David investigated mechanisms to improve the corrosion resistance of magnesium alloys for use as a biomaterial for skeletal implants (e.g., fixators and bone screws). Through laser processing, it was possible to tune the surface chemistry of magnesium implants to change their degradation rate in simulated body fluid. This research was conducted at the University of Virginia and Monash University.
- D. Florian, M. Odziomek, C. Ock, H. Chen, and S. Guelcher. Principles of computer-controlled linear motion applied to an open-source affordable liquid handler for automated micropipetting. Scientific Reports (2020)
- D. Florian, G. Lowen, K. Kwakwa, J. Vanderburgh, and J. Rhoades. Biomaterials and Tissue Engineering Cancer Models. Encyclopedia of Bone Biology (2019)
- D. Florian, M. Melia, F. Steuer, and M. Purzychi. Laser Irradiation of Mg-Al-Zn alloy: Reduced Electrochemical Kinetics and Enhanced Performance in Simulated Body Fluid. Biointerphases (2017)
- M. Melia, D. Florian, et al. Investigation of critical processing parameters for laser surface processing of AZ31B-H24. Surface and Coatings Technology (2017)
- M. Melia, M. Serron, D. Florian, J. Weiler, J. Scully and J. Fitz-Gerald. Excimer laser processing of Al containing Mg alloys for improved corrosion resistance
A Renaissance Man
David prides himself on his diverse skill set. His most prominent fields of expertise are additive manufacturing (i.e., 3D printing) and biotechnology. Below is a list of his technical skills.
Digital Fabrication is a design and manufacturing workflow, where parts are created digitally within a CAD (computer-aided design) program prior to being transferred over to CAM (computer-aided manufacturing) software. The CAM software outputs code that instructs digital fabrication tools, which additively (e.g., 3D print) or subtractively (e.g., mill) manufacture parts. David is not only fluent in CAD programs, such as Fusion 360 and SolidWorks, but is also well-versed in designing toolpaths in Inventor HSM and Fusion 360 CAM.
David can operating a number of different computer controlled (CNC) fabrication tools to turn 3D models into real-life parts. David has experience with the following additive manufacturing technologies:
- Fused Filament Fabrication (FFF)
- Stereolithography (SLA)
- Selective Laser Sintering (SLS)
- 3D Inkjet Printing
David can also run the following subtractive manufacturing tools:
- 4-Axis CNC Mill
- CNC Plasma Cutter
- Laser Cutter
David has the ability to program in C/C++ and python. Most of his programming skills have evolved from performing computational analyses in his research. However, he has some experience in developing desktop applications with Node.js/Electron for CNC control.
Most of David’s research occurs at the cellular level, and consequently, a microscope is required to interrogate the complex phenomena of cancer metastasis and immune activation. David is a trained operator of the following types of microscopes:
- Scanning Electron Microscope (SEM)
- Epifluorescent Microscope
- Confocal Microscope (Single and multi- photon scopes)
David’s understanding of optical systems has applications to many different fields, including photonics/laser science.
David routinely performs biological assays to measure the effects of perturbations on his cancer models. While David has the ability to follow the protocol for any number of biological assay, below is a list of assays that he is proficient at:
- Flow Cytometry
- Quantitative Polymerase Chain Reaction (qPCR)
- Western Blot
- Immunohistochemistry (IHC)
- Immunofluorescence (IF)
While David has no formal training in video filming and editing. The success of his YouTube channel highlights his ability to distill complex topics into entertaining and informative videos.